| 產(chǎn)品編號(hào) | bs-0624R |
| 英文名稱 | CD272 Rabbit pAb |
| 中文名稱 | B和T淋巴細(xì)胞衰減蛋白抗體 |
| 別 名 | B and T lymphocyte associated protein; B and T lymphocyte attenuator; B and T lymphocyte associated; BTLA; BTLA1; CD272 antigen; FLJ16065; MGC129743; BTLA_HUMAN; B-and T-lymphocyte attenuator; B-and T-lymphocyte-associated protein. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 淋巴細(xì)胞 t-淋巴細(xì)胞 b-淋巴細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human B and T-lymphocyte attenuator: 221-289/289 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
B and T lymphocyte attenuator (BTLA), an immunoglobulin domain-containing glycoprotein with two immunoreceptor tyrosine-based inhibitory motifs. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. Crosslinking BTLA with antigen receptors induces its tyrosine phosphorylation and association with the Src homology domain 2 (SH2)-containing protein tyrosine phosphatases SHP-1 and SHP-2, and attenuates production of interleukin 2 (IL-2). BTLA-deficient T cells show increased proliferation, and BTLA-deficient mice have increased specific antibody responses and enhanced sensitivity to experimental autoimmune encephalomyelitis. B7x, a peripheral homolog of B7, is a ligand of BTLA. Thus, BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1). Function: Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response. Subunit: Interacts with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. Interacts with TNFRSF14/HVEM. Subcellular Location: Membrane; Single-pass type I membrane protein (Potential). Post-translational modifications: Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11. N-glycosylated. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q7Z6A9 Gene ID: 151888 Database links: Entrez Gene: 151888 Human Entrez Gene: 208154 Mouse Omim: 607925 Human SwissProt: Q7Z6A9 Human SwissProt: Q7TSA3 Mouse Unigene: 445162 Human Unigene: 38199 Mouse Unigene: 124474 Rat BTLA對(duì)T細(xì)胞的活化、增殖起著重要的負(fù)調(diào)控作用，BTLA相應(yīng)配體為TNFR超家族中的皰疹病毒入侵介質(zhì)（HVEM）, 其表達(dá)于包括T細(xì)胞在內(nèi)的多種免疫細(xì)胞表面。 有學(xué)者將他定為CD28的超級(jí)族成員，B、T淋巴細(xì)胞衰減子-CD272主要用于細(xì)胞信號(hào)傳導(dǎo)方面的研究。 近來國(guó)外學(xué)者對(duì)BTLA用于抑制腫瘤方面的研究也有了新的進(jìn)展，認(rèn)為B、T淋巴細(xì)胞衰減子對(duì)腫瘤的生長(zhǎng)有抑制作用，探索新的腫瘤免疫治療有了新的途徑， 封閉此途徑有可能成為腫瘤免疫治療的新靶點(diǎn)。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Rat Thymus tissue lysates
Lane 2: Rat Lung tissue lysates
Primary: Anti-CD272 (bs-0624R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 55 kDa
Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-CD272/BTLA Polyclonal Antibody, Unconjugated(bs-0624R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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