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Orexin receptor 1+2 Rabbit pAb (bs-1095R)  
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產(chǎn)品編號 bs-1095R
英文名稱 Orexin receptor 1+2 Rabbit pAb
中文名稱 丘腦分泌素受體1+2/食欲素受體1+2抗體
別    名 Orexin Receptor 1 and 2; Orexin receptor 1+2; Orexin Receptor 2; HCRTR 1; HCRTR1; Hypocretin receptor type 1; Orexin receptor type 1; Ox-1-R; Ox1-R; Ox1r; OX1R_HUMAN; Hcrtr2; Hypocretin(orexin) receptor 2; Hypocretin receptor 2; Hypocretin receptor type 2  
Specific References  (1)     |     bs-1095R has been referenced in 1 publications.
[IF=12.121] Hui Fenget al. Orexin signaling modulates synchronized excitation in the sublaterodorsal tegmental nucleus to stabilize REM sleep. Nat Commun . 2020 Jul 21;11(1):3661.  IHC ;  Rats , mice.  
研究領(lǐng)域 神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)  通道蛋白  細(xì)胞膜受體  內(nèi)分泌病  G蛋白偶聯(lián)受體  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 50 kDa
檢測分子量
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Orexin receptor 1/2: 321-425/444 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein binds the hypothalamic neuropeptides orexin A and orexin B. A related gene (HCRTR1) encodes a G-protein coupled receptor that selectively binds orexin A. [provided by RefSeq, Jan 2009]

Function:
Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
O43614

Gene ID:
3062

Database links:

Entrez Gene: 3061 Human

Entrez Gene: 230777 Mouse

Entrez Gene: 25593 Rat

Omim: 602392 Human

SwissProt: O43613 Human

SwissProt: P58307 Mouse

SwissProt: P56718 Rat

Unigene: 388226 Human

Unigene: 246595 Mouse

Unigene: 88262 Rat

Entrez Gene: 3062 Human

Entrez Gene: 387285 Mouse

Entrez Gene: 25605 Rat

Omim: 602393 Human

SwissProt: O43614 Human

SwissProt: P58308 Mouse

SwissProt: P56719 Rat

Unigene: 151624 Human

Unigene: 699147 Human

Unigene: 335300 Mouse

Unigene: 9893 Rat



食欲素受體(OXR)Orexin receptor—是一種G蛋白偶合受體. 下丘腦是調(diào)節(jié)飲食及能量平衡的中樞.學(xué)者認(rèn)為:在下丘腦可促進(jìn)食欲且來源于同一前體的兩種新神經(jīng)肽:食欲素A和B,它們可激活兩種密切相關(guān)且與G蛋白偶聯(lián)的細(xì)胞表面受體(OX1R,OX2R).含Orexin的神經(jīng)細(xì)胞在下丘腦腹外側(cè)呈對稱的不連貫分布. 在飲食反饋調(diào)節(jié)中具有重要作用.食欲素與其它影響進(jìn)食的神經(jīng)肽之間存在錯(cuò)綜復(fù)雜的關(guān)系.它在增加攝食、飲水、調(diào)節(jié)睡眠覺醒周期、生殖、體溫、血壓和感覺等方面有廣泛作用.
產(chǎn)品圖片
Sample: Cerebral cortex (Mouse) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Brain (Mouse) Lysate at 40 ug Brain (Rat) Lysate at 40 ug Primary: Anti- Orexin receptor 1+2 (bs-1095R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Rat Cerebellum tissue lysates Primary: Anti-Orexin receptor 1+2 (bs-1095R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse Cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Orexin receptor 1+2) Polyclonal Antibody, Unconjugated (bs-1095R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Orexin receptor 1+2) Polyclonal Antibody, Unconjugated (bs-1095R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD200R/Orexin receptor Polyclonal Antibody, Unconjugated(bs-1095R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD200R/Orexin receptor Polyclonal Antibody, Unconjugated(bs-1095R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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