| 產品編號 | bs-0449R |
| 英文名稱 | TSLP Rabbit pAb |
| 中文名稱 | 胸腺基質淋巴細胞生成素-1抗體 |
| 別 名 | Thymic stromal lymphopoietin; Thymic stromal lymphopoietin isoform 1; Thymic stromal lymphopoietin protein TSLP; TSLP protein; TSLP_HUMAN. |
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Specific References (4) | bs-0449R has been referenced in 4 publications.
[IF=5.34] Dian-Dong Hou. et al. Therapeutic effects of myricetin on atopic dermatitis in vivo and in vitro. PHYTOMEDICINE. 2022 Jul;102:154200 IHC ; Mouse.
[IF=3.483] Shen?Z et al. Insights into Roseburia intestinalis which alleviates experimental colitis pathology by inducing anti-inflammatory responses. J Gastroenterol Hepatol. 2018 Oct;33(10):1751-1760. IHC-P ; Mouse.
[IF=2.493] Min-jie Gong. et al. Melatonin reduces IL-33 and TSLP expression in human nasal epithelial cells by scavenging ROS directly. IMMUN INFLAMM DIS. 2023 Feb;11(2):e788 WB ; Human.
[IF=0.67] Sakitani, Eri, et al. "Increased Expression of Thymic Stromal Lymphopoietin and Its Receptor in Kimuras Disease." ORL 77.1 (2015): 44-54. other ;
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| 研究領域 | 細胞生物 免疫學 細胞因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TSP-1: 101-159/159 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Thymic stromal lymphopoietin (TSLP) is a novel cytokine that was found to promote the development of murine B cells in vitro. This protein, which is expressed in a number of tissues including heart, liver and prostate, prevented apoptosis and stimulated growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ-3. Anti-interleukin (IL)-7 receptor antibodies (Abs) neutralized this effect indicating that TSLP binds to at least part of the IL-7 receptor complex. TSLP induced phosphorylation of signal transducer and activator of transcription (STAT)-5. Function: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. Subunit: Interacts with a receptor composed of CRLF2 and IL7R. Subcellular Location: Secreted. Tissue Specificity: Expressed in a number of tissues including heart, liver and prostate. SWISS: Q969D9 Gene ID: 85480 Database links: Entrez Gene: 85480 Human Entrez Gene: 53603 Mouse Omim: 607003 Human SwissProt: Q969D9 Human SwissProt: Q9JIE6 Mouse Unigene: 389874 Human Unigene: 143716 Mouse Unigene: 201724 Rat 胸腺基質淋巴細胞生成素-1（胸腺基質細胞分泌的淋巴細胞生成素-1） 為一種新型促進生長的細胞因子——胸腺基質淋巴蛋白（TSLP）可以引發(fā)炎癥反應， 這種炎癥反應可以引起很多過敏性疾病和哮喘。 TSLP可能是過敏性疾病最重要的引發(fā)劑，已得到科學家的重視，如果證實了 TSLP是一種起始因子，抑制TSLP可以有效地治療哮喘和其它過敏性疾病。 |
| 產品圖片 |
Sample:
Lane1:K562 Cell Lysate at 30ug
Lane2: Raji Cell Lysate at 30ug
Primary: Anti-TSLP (bs-0449R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size: 15 kD
Observed band size: 17 kD
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TSLP Polyclonal Antibody, Unconjugated(bs-0449R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TSLP) Polyclonal Antibody, Unconjugated (bs-0449R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TSLP) Polyclonal Antibody, Unconjugated (bs-0449R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
