| 產(chǎn)品編號(hào) | bs-2455R |
| 英文名稱 | CLEC7A Rabbit pAb |
| 中文名稱 | β葡聚糖受體抗體 |
| 別 名 | CLC7A_HUMAN; C-type lectin domain family 7 member A; BGR; beta GR; CLECSF12; DECTIN1; beta-glucan receptor; beta Glucan Receptor; C-type lectin superfamily member 12; C type calcium dependent carbohydrate recognition domain lectin superfamily member 12; D |
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Specific References (5) | bs-2455R has been referenced in 5 publications.
[IF=19.227] Iván Ballasch. et al. Ikzf1 as a novel regulator of microglial homeostasis in inflammation and neurodegeneration. BRAIN BEHAV IMMUN. 2023 Mar;109:144 WB ; Mouse.
[IF=4.784] Cui H et al. A β-glucan from Grifola frondosa effectively delivers therapeutic oligonucleotide into cells via dectin-1 receptor and attenuates TNFα gene expression. Int J Biol Macromol. 2020 Jan 23;149:801-808. WB ; Mouse.
[IF=4.784] Zhan Q et al. Structural Characterization and Immunomodulatory Activity of a Novel Acid Polysaccharide Isolated from the Pulp of Rosa laevigata Michx Fruit. Int J Biol Macromol. 2019 Nov 12. pii: S0141-8130(19)37108-9. Other ; Mouse.
[IF=4.012] Wu J et al. Paeonol ameliorates murine alcohol liver disease via mycobiota‐mediated Dectin‐1/IL‐1β signaling pathway. J Leukoc Biol. 2020 Mar 4. WB ; mouse.
[IF=1.16] Li et al. Expression of dectin-1 during fungus infection in human corneal epithelial cells. (2014) Int.J.Ophthalmol. 7:34-7 IHC ; Human.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 淋巴細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CLEC7A: 41-140/247 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq]. Function: Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation (By similarity). Subunit: Homodimer. Interacts with SYK; participates in leukocyte activation in presence of fungal pathogens. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Tissue Specificity: Detected in dendritic cells, in paracortical and medullary regions of lymph nodes, and in spleen red pulp and white pulp. Post-translational modifications: on tyrosine residues in response to glucan binding. Similarity: Contains 1 C-type lectin domain. SWISS: Q9BXN2 Gene ID: 64581 Database links: Entrez Gene: 64581 Human Entrez Gene: 56644 Mouse Omim: 606264 Human SwissProt: Q9BXN2 Human SwissProt: Q6QLQ4 Mouse Unigene: 143929 Human Unigene: 239516 Mouse β-葡聚糖受體是一種非調(diào)理素依賴性受體,可在免疫反應(yīng)早期,在清除衰老細(xì)胞和防御感染方面發(fā)揮重要作用,進(jìn)而實(shí)現(xiàn)其抗腫瘤、抗感染等生物學(xué)效應(yīng)。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Lung tissue lysates
Primary: Anti-beta Glucan Receptor (bs-2455R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28,47 kDa
Sample:
Spleen (Mouse) Lysate at 40 ug
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- beta Glucan Receptor (bs-2455R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Sample: Lung(Mouse) lysate at 30ug;
Primary: Anti-beta Glucan Receptor (bs-2455R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1:5000 dilution;
Predicted band size: 28 kD
Observed band size: 28 kD
Blank control:RAW264.7.
Primary Antibody (green line): Rabbit Anti-Iba1 antibody (bs-2455R)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-Rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:THP-1.
Primary Antibody (green line): Rabbit Anti-beta Glucan Receptor antibody (bs-2455R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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