| 產(chǎn)品編號 | bs-10016R |
| 英文名稱 | phospho-ZCWCC1 (Ser739) Rabbit pAb |
| 中文名稱 | 磷酸化ZCWCC1抗體 |
| 別 名 | AC004542.C22.1.; p-MORC2(Ser739); phospho-ZCWCC1(Ser739); CW type with coiled coil domain 1; KIAA0852; ZCW3; ZCWCC1; Zinc finger; Zinc finger CW type coiled coil domain protein 1; Zinc finger CW type with coiled coil domain 1; Zing finger CW type 3 zinc f |
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Specific References (1) | bs-10016R has been referenced in 1 publications.
[IF=5.17] Wang et al. PAK1-mediated MORC2 phosphorylation promotes gastric tumorigenesis. (2015) Oncotarge. 6:9877-86 WB, IHC-P ; Human, Mouse.
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| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 細胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 114 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human MORC2 around the phosphorylation site of Ser739: KR(p-S)VA |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The CW domain is a structural module found in many vertebrate, parasitic and plant proteins. It consists of a mononuclear four-cysteine zinc-finger domain that plays a role in DNA binding, chromatin methylation and early embryonic development. ZCWCC1 (zinc finger CW-type coiled-coil domain protein 1), also known as MORC2 (MORC family CW-type zinc finger protein 2) or ZCW3, is a 1,032 amino acid protein that contains one CW-type zinc finger domain. ZCWCC1 is located on chromosome 22 and is ubiquitously expressed with highest expression in pancreas, smooth muscle and testis. Expression of ZCWCC1 is upregulated in hypoxia, a pathological condition characterized by an inadequate supply of oxygen in the blood. Function: May act as a transcriptional repressor. Down-regulates CA9 expression. Subunit: Interacts with HDAC4. Subcellular Location: Nucleus. Cytoplasm, cytosol. Note=Mainly located in the nucleus. Tissue Specificity: Highly expressed in smooth muscle, pancreas and testis. Similarity: Contains 1 CW-type zinc finger. SWISS: Q9Y6X9 Gene ID: 22880 Database links: Entrez Gene: 22880 Human Entrez Gene: 74522 Mouse SwissProt: Q9Y6X9 Human Unigene: 143840 Human Unigene: 555918 Human Unigene: 139127 Mouse |
| 產(chǎn)品圖片 |
Sample:
MCF-7(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-ZCWCC1 (Ser739) (bs-10016R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 114 kD
Observed band size: 114 kD
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-ZCWCC1 (Ser739)) Polyclonal Antibody, Unconjugated (bs-10016R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-ZCWCC1(Ser739) Polyclonal Antibody, Unconjugated(bs-10016R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-ZCWCC1 (Ser739)) polyclonal Antibody, Unconjugated (bs-10016R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
