| 產(chǎn)品編號 | bs-4897R |
| 英文名稱 | ACKR3 Rabbit pAb |
| 中文名稱 | 細胞表面趨化因子受體7抗體 |
| 別 名 | GPCR RDC1; C X C chemokine receptor type 7; Chemokine C X C motif receptor 7; Chemokine orphan receptor 1; CMKOR1; CXC R7; CXCR 7; CXCR7; G protein coupled receptor 159; G protein coupled receptor RDC1 homolog; GPR159; RDC 1; RDC1; CXCR7_HUMAN. |
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Specific References (3) | bs-4897R has been referenced in 3 publications.
[IF=2.93] Tang, Xin, et al. "Downregulation of CXCR7 inhibits proliferative capacity and stem cell-like properties in breast cancer stem cells." Tumor Biology (2016): 1-9. WB ; Human.
[IF=1.39] Cao et al. MicroRNA-100 suppresses human gastric cancer cell proliferation by targeting CXCR7. (2018) Oncol.Lett. 15:453-458 WB ; Human.
[IF=1.39] Cao, Yongfeng, et al. "MicroRNA-100 suppresses human gastric cancer cell proliferation by targeting CXCR7." Oncology Letters. WB ; Human.
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| 研究領(lǐng)域 | 血管內(nèi)皮細胞 b-淋巴細胞 內(nèi)皮細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 細胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CXCR7: 151-250/362 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. GCPR RDC1 is reported to be expressed in human cell lines and in cultured vascular endothelial cells and in rodent brain, kidney, lung, heart, spleen, pancreas, small intestine, blood, colon, and blood vessels. ESTs have been isolated from human normal bone marrow, brain, breast, cartilage, embryo, eye, fetal lung/testis/B-cell, heart, heart/melanocyte/uterus, kidney, liver/spleen, nerve, placenta, and umbilical libraries as well as several human cancer libraries. Function: Receptor for chemokines CXCL12/SDF1 and CXCL11. Does not elicit classical chemokine receptor signaling; chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Acts as a scavenger for CXCL12/SDF1 and, to a lesser extent, for CXCL11. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal haematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Subunit: Homodimer. Can form heterodimers with CXCR4; heterodimerization may regulate CXCR4 signaling activity. Subcellular Location: Cell membrane; Multi-pass membrane protein. Cytoplasm, perinuclear region. Early endosome. Recycling endosome. Note=Localized mainly in perinuclear regions in neurons and in early endosomes in T-lymphocytes and some other cell types, with very low levels detected on the cell surface. May spontaneously cycle between the plasma membrane and endosomal compartments. Tissue Specificity: Expressed in monocytes, basophils, B-cells, umbilical vein endothelial cells (HUVEC) and B-lymphoblastoid cells. Lower expression detected in CD4+ T-lymphocytes and natural killer cells. In the brain, detected in endothelial cells and capillaries, and in mature neurons of the frontal cortex and hippocampus. Expressed in tubular formation in the kidney. Highly expressed in astroglial tumor endothelial, microglial and glioma cells. Expressed at low levels in normal CD34+ progenitor cells, but at very high levels in several myeloid malignant cell lines. Expressed in breast carcinomas but not in normal breast tissue (at protein level). Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P25106 Gene ID: 57007 Database links: Entrez Gene: 57007 Human Entrez Gene: 12778 Mouse Omim: 610376 Human SwissProt: P25106 Human SwissProt: P56485 Mouse Unigene: 471751 Human Unigene: 6522 Mouse Unigene: 12959 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ACKR3) Polyclonal Antibody, Unconjugated (bs-4897R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CXCR7/RDC1 Polyclonal Antibody, Unconjugated(bs-4897R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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