| 產(chǎn)品編號 | bs-19178R |
| 英文名稱 | PRKCBP1 Rabbit pAb |
| 中文名稱 | 蛋白激酶C結(jié)合蛋白1抗體 |
| 別 名 | ZMYND8; CTCL tumor antigen se14 3; Cutaneous T cell lymphoma associated antigen se14 3; KIAA1125; MGC31836; predicted protein of HQ2893; PRKCBP 1; PKCB1_HUMAN; PRO2893; Protein kinase C binding protein 1; Rack7; Zinc finger MYND domain containing protein |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 激酶和磷酸酶 結(jié)合蛋白 淋巴細胞 t-淋巴細胞 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rabbit,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 119 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PRKCBP1: 1-100/1186 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Subunit: Interacts in vitro with PRKCB1. Subcellular Location: Nuclear Tissue Specificity: Expressed in all tissues examined with highest expression in brain, lung, pancreas, and placenta. Expressed in cutaneous T-cell lymphomas (CTCL). Similarity: Contains 1 bromo domain. SWISS: Q9ULU4 Gene ID: 23613 Database links: Entrez Gene: 23613 Human SwissProt: Q9ULU4 Human Unigene: 446240 Human |
| 產(chǎn)品圖片 |
Sample:
A549(Human) Cell Lysate at 40 ug
Primary: Anti-PRKCBP1 (bs-19178R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 119 kD
Observed band size: 119 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRKCBP1) Polyclonal Antibody, Unconjugated (bs-19178R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
