| 產品編號 | bs-33011R |
| 英文名稱 | RFP Rabbit pAb |
| 中文名稱 | 紅色熒光蛋白單克隆抗體 |
| 別 名 | Red fluorescent protein; Red fluorescent protein eqFP611; RFPTag; RFP-Tag; RFP Tag; RFP_ENTQU. |
| 產品類型 | 標簽抗體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Recombinant protein |
| 產品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant Red fluorescent protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Red Fluorescent Protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm. |
| 產品圖片 |
Sample:
Lane 1: Recombinant RFP protein, His
Lane 2: Tags Positive Control Whole Cell Lysate, C-His
Primary: Anti-RFP (bs-33011R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: kDa
Observed band size: 30,44 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Human RFP protein, His Tag (Cat. bs-33011P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human RFP Antibody, the EC50 is 147.7 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human RFP protein, His Tag (Cat. bs-33011P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human RFP Antibody, the EC50 is 161.8 ng/mL.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
