| 產(chǎn)品編號(hào) | bsm-60085M |
| 英文名稱 | Di/Tri-Methyl-Histone H4 (Lys20) Recombinant Mouse mAb |
| 中文名稱 | |
| 產(chǎn)品類(lèi)型 | 甲基化抗體 |
| 抗體來(lái)源 | Mouse |
| 克隆類(lèi)型 | Recombinant |
| 克 隆 號(hào) | G9B11 |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 蛋白質(zhì)的ε-氨基賴氨酸乙?；强刂频鞍踪|(zhì)活性的一種重要的可逆修飾。核心組蛋白的氨基末端在多個(gè)位點(diǎn)發(fā)生賴氨酸甲基化，稱為“組蛋白密碼”或“表觀遺傳密碼”。核心組蛋白中賴氨酸甲基化是形成基因組活性區(qū)和非活性區(qū)的主要決定因素，因此在多種細(xì)胞事件中起著至關(guān)重要的作用。在大多數(shù)物種中，賴氨酸甲基化主要發(fā)生在組蛋白H3（Lys4、9、27、36、79）和H4（Lys20）上，并且與轉(zhuǎn)錄激活和沉默有關(guān)。受組蛋白甲基化酶（HMTs）和組蛋白去甲基化酶（HDMs）調(diào)控的組蛋白甲基化在癌癥和其他疾病的病理過(guò)程中受到損害，因此，調(diào)節(jié)組蛋白賴氨酸甲基化的酶已成為抗癌藥物的靶點(diǎn)。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, N2a, BRL, Mouse spleen, Mouse kidney, rH4
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Human neuroblastoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60085M
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