| 產(chǎn)品編號 | bs-25450R |
| 英文名稱 | phospho-DDX5 (Tyr593) Rabbit pAb |
| 中文名稱 | 磷酸化三磷酸腺苷依賴解旋酶ddx5抗體 |
| 別 名 | DDX5(phospho Y593); DDX5(phospho Tyr593); p-DDX5(Y593); p-DDX5(Tyr593); ATP dependent RNA helicase DDX5; DDX 5; Ddx5; DDX5_HUMAN; DEAD(Asp Glu Ala Asp) box helicase 5; DEAD(Asp Glu Ala Asp) box polypeptide 5; DEAD box 5; DEAD box protein 5; DEAD/H(Asp Glu |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Cow,Horse) |
| 產(chǎn)品應用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 69 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human DDX5 around the phosphorylation site of Tyr593: QA(p-Y)AY |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
p68 RNA helicase is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA helicase activity. p68 RNA helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA helicase increases in serum-induced quiescent cell lines. p68 RNA helicase may function as a coactivator for estrogen receptor alpha. Additionally, p68 RNA helicase associates with transcriptional coactivators CBP and p300. p68 RNA helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA helicase and fibrillarin colocalize to nascent nucleoli. p68 RNA helicase may function as a heterodimer with p72 RNA helicase. Function: RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing. Subunit: Interacts with BRDT (By similarity). Identified in thespliceosome C complex. Interacts with RBM4; the interaction occursin a RNA-independent manner. Interacts with EIF2C1 and EIF2C2.Interacts with ESR1; the interaction is enhanced by phosphorylationof ESR1 AF-1 domain. Interacts with AR, NCOA1, NCOA2, NCOA3, EP300,CREBBP, POLR2A, TP53, RUNX2 and HDAC1. Self-associates. Interactswith DDX17. [INTERACTION] Q12873:CHD3; NbExp=4; IntAct=EBI-351962, EBI-523590;P45481:Crebbp (xeno); NbExp=3; IntAct=EBI-351962, EBI-296306;Q09472:EP300; NbExp=4; IntAct=EBI-351962, EBI-447295; P03372:ESR1;NbExp=8; IntAct=EBI-351962, EBI-78473; P22087:FBL; NbExp=6;IntAct=EBI-351962, EBI-358318; Q13547:HDAC1; NbExp=4;IntAct=EBI-351962, EBI-301834; O95983:MBD3; NbExp=4;IntAct=EBI-351962, EBI-1783068; P10085:Myod1 (xeno); NbExp=3;IntAct=EBI-351962, EBI-4405734; P24928:POLR2A; NbExp=3;IntAct=EBI-351962, EBI-295301; Q08775-3:Runx2 (xeno); NbExp=2;IntAct=EBI-351962, EBI-6119991; P04637:TP53; NbExp=3;IntAct=EBI-351962, EBI-366083; P04637-1:TP53; NbExp=2;IntAct=EBI-351962, EBI-3895849; P04637-7:TP53; NbExp=2;IntAct=EBI-351962, EBI-3895873. Subcellular Location: Nucleus. Post-translational modifications: Arg-502 is dimethylated, probably to asymmetricdimethylarginine. Sumoylated; sumoylation, promoted by PIAS1, promotesinteraction with HDAC1 and transcriptional repression activity.Sumoylation also significantly increases stability, and reducespolyubiquitination. Polyubiquitinated, leading to proteasomal degradation. Similarity: Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SWISS: P17844 Gene ID: 1655 Database links: Entrez Gene: 1655 Human Entrez Gene: 13207 Mouse Omim: 180630 Human SwissProt: P17844 Human SwissProt: Q61656 Mouse Unigene: 279806 Human Unigene: 220038 Mouse Unigene: 162208 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human MCF-7 cell lysates
Primary: Anti-phospho-DDX5 (Tyr593) (bs-25450R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 65 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
